For sharp intracellular microelectrodes, add additional weights to increase the pulling force during the final stage. Step 3: Configuring Pull Parameters
Set the digital heat controls for (first pull/shaping) and Heater No. 2 (second pull/terminal tip formation). Note: Refer to your lab's specific protocol for baseline values, typically starting between 60 and 80 on the dial. Step 2: Loading the Glass Capillary Loosen the upper and lower pipette clamps.
Carefully remove the upper and lower pipettes vertically to avoid snapping the fragile tips.
: Includes two light (approx. 25g) and two heavy (approx. 100g) weights for fine-tuning tension. narishige pc10 manual new
If you are looking for the original Narishige PC-10 Manual , it is still hosted by distributors like ALA Scientific Instruments for legacy support. For those upgrading, the PC-100 Product Page at Narishige Group provides documentation for the current standard.
Lower the acrylic safety shield. Press the button. The coil will glow, the glass will soften, and gravity will drop the lower clamp to create your micropipettes. 5. Calibration and Finding the Filament Burn Value
She’d inherited the controller and the manual from Dr. Sato, who’d guided her through her first awkward hours at the bench. He’d called it “the storyteller of precision,” and Lena had never forgotten that phrase. Tonight, alone, she wanted to learn it herself—every button, every whisper of function—and perhaps to discover why the lab’s coffee machine broke exactly the day Sato left. Note: Refer to your lab's specific protocol for
Rotate the bottom switch 90 degrees counterclockwise to the "under weight" position (if applicable to your version). Press the red Start Switch
The heater coil becomes extremely hot during operation. Never touch the coil while the unit is powered on.
Place the unit away from air conditioning vents, windows, and heavy foot traffic. Drafts alter filament cooling times, causing inconsistent pipette shapes. : Includes two light (approx
You will also need to order consumable glass capillaries. The compatible glass types include:
Note: If by "PC10" you actually meant the PC-10 hydraulic micromanipulator or a specific accessory part, please clarify, but the above review applies to their most popular item bearing that model designation.
Clean your glass capillaries with ethanol and dry them thoroughly to remove finger oils and dust. Dust particles cause asymmetric breaks during the pull. Step 2: Install the Capillary