Shimadzu: Spd20a User Manual
Install a backpressure regulator on the waste line to prevent bubble formation inside the cell.
Connect the tubing from the "OUT" port of the flow cell to your waste container or next in-line detector (e.g., a Mass Spectrometer or Refractive Index detector).
Contaminated flow cells cause baseline drift and ghost peaks. Flush the cell with HPLC-grade water to remove salts. Flush with 1M Nitric Acid ( HNO3cap H cap N cap O sub 3 ) if organic fouling is severe. shimadzu spd20a user manual
Turn off the detector power, unplug the power cord, and allow the lamp to cool for at least 30 minutes to avoid severe skin burns.
Avoid touching the glass envelope of the new lamp with bare hands (skin oils create hot spots that crack the glass). Use lint-free gloves. Align the positioning pin, insert the lamp, and tighten the screws. Install a backpressure regulator on the waste line
Connect the waste tubing to the flow cell outlet port (typically the top port).
This comprehensive guide serves as an practical manual for operators, covering installation, daily usage, routine maintenance, and troubleshooting. 1. Technical Overview and Specifications Flush the cell with HPLC-grade water to remove salts
The lists several troubleshooting steps for common problems: Potential Cause Noisy Baseline Air bubbles in cell, dirty lamp, improper temperature Flush cell, replace lamp, check lamp temperature settings. Constant Drift Temperature fluctuation, leaking solvent Stabilize room temp, check fittings for leaks. No Signal Lamp off, lamp dead, improper wavelength Check lamp power, set proper wavelength. Error Message (e.g., E001) Wavelength calibration error Contact service or restart unit. 6. Accessing the Official Shimadzu SPD-20A User Manual
When the lamp noise increases or light intensity decreases, the Deuterium ( D2cap D sub 2
